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After immunostaining, eWAT sections were mounted with fluorescent mounting medium and images were taken using a confocal microscope LSM710

Cells were then washed and stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen, 1:1000) for 30 min

Possible causes include brain infection (viral encephalitis, subacute sclerosing panencephalitis, Whipple's disease) or inflammation (limbic encephalitis, Hashimoto's encephalopathy, cerebral vasculitis)

Patients are often seen by a variety of health care providersin the emergency room, urgent care clinics, and pediatricians officesso the cyclic pattern goes unnoticed until a parent becomes concerned

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Cancer, the second leading cause of U.S